Journal: Revista da Sociedade Brasileira de Medicina Tropical

Article Title: Role of transforming growth factor-beta 1 and connective tissue growth factor levels in coronavirus disease-2019-related lung Injury: a prospective, observational, cohort study

doi: 10.1590/0037-8682-0615-2021

Figure Lengend Snippet: Study groups’ TGF-β1, CTGF, and other laboratory parameter results

Article Snippet: TGF-β1 and CTGF values were determined using the ELISA method with a Human TGF-β1 ELISA kit (Elabscience, Human TGF-β1: cat. E-EL-H0110, Texas, USA) and a human CTGF ELISA kit (Elabscience, Human CTGF: E-EL-H0828, Texas, USA) in accordance with the manufacturer’s recommendations.

Techniques:

Journal: Revista da Sociedade Brasileira de Medicina Tropical

Article Title: Role of transforming growth factor-beta 1 and connective tissue growth factor levels in coronavirus disease-2019-related lung Injury: a prospective, observational, cohort study

doi: 10.1590/0037-8682-0615-2021

Figure Lengend Snippet: Box-plot presentation of serum TGF-β1 and CTGF levels on day 7 of admission in Group 1 divided by severity of COVID-19 disease. *:p<0.001 (analysis of variance) two-way comparison of subgroups.

Article Snippet: TGF-β1 and CTGF values were determined using the ELISA method with a Human TGF-β1 ELISA kit (Elabscience, Human TGF-β1: cat. E-EL-H0110, Texas, USA) and a human CTGF ELISA kit (Elabscience, Human CTGF: E-EL-H0828, Texas, USA) in accordance with the manufacturer’s recommendations.

Techniques: Comparison

Journal: Revista da Sociedade Brasileira de Medicina Tropical

Article Title: Role of transforming growth factor-beta 1 and connective tissue growth factor levels in coronavirus disease-2019-related lung Injury: a prospective, observational, cohort study

doi: 10.1590/0037-8682-0615-2021

Figure Lengend Snippet: Changes in serum TGF-β1 and CTGF levels among disease severity subgroups.

Article Snippet: TGF-β1 and CTGF values were determined using the ELISA method with a Human TGF-β1 ELISA kit (Elabscience, Human TGF-β1: cat. E-EL-H0110, Texas, USA) and a human CTGF ELISA kit (Elabscience, Human CTGF: E-EL-H0828, Texas, USA) in accordance with the manufacturer’s recommendations.

Techniques:

Journal: Revista da Sociedade Brasileira de Medicina Tropical

Article Title: Role of transforming growth factor-beta 1 and connective tissue growth factor levels in coronavirus disease-2019-related lung Injury: a prospective, observational, cohort study

doi: 10.1590/0037-8682-0615-2021

Figure Lengend Snippet: Determination of the diagnostic sensitivity and specificity of serum TGF-β1 and CTGF levels on days 1 and 7 of admission in patients diagnosed with COVID-19 using ROC curve analysis. ROC: receiver-operating characteristic curve.

Article Snippet: TGF-β1 and CTGF values were determined using the ELISA method with a Human TGF-β1 ELISA kit (Elabscience, Human TGF-β1: cat. E-EL-H0110, Texas, USA) and a human CTGF ELISA kit (Elabscience, Human CTGF: E-EL-H0828, Texas, USA) in accordance with the manufacturer’s recommendations.

Techniques: Diagnostic Assay

Journal: Nature Communications

Article Title: Role of CTGF-LRP1 in impaired healing of cesarean section incisions

doi: 10.1038/s41467-026-69747-9

Figure Lengend Snippet: a Schematic of experimental groups: fibroblasts derived from Adjacent, Control, and Niche tissues were treated with recombinant human CTGF (rhCTGF). b Western blot analysis of Collagen I and III in fibroblasts following rhCTGF treatment. c Schematic of transcriptomic analysis in human uterine fibroblasts (HUFs) with modulated LRP1 expression under rhCTGF stimulation. d Collagen I and III expression in HUFs transfected with control siRNA (siNC) or LRP1 siRNA (siLRP1) and stimulated with rhCTGF. e Volcano plot showing differentially expressed genes (DEGs) between rhCTGF-treated siNC and siLRP1 HUFs. f GO analysis indicating suppression of ERK and WNT pathways in LRP1-silenced, rhCTGF-treated HUFs. g Western blot of phosphorylated ERK in siNC- or siLRP1-transfected HUFs treated with rhCTGF. h Phospho-ERK levels in Adjacent, Control, and Niche fibroblasts treated with rhCTGF. i Western blot analysis of Collagen I, Collagen III, and phospho-β-catenin in HUFs transfected with siNC or siLRP1 and treated with rhCTGF in combination with PD98059 (MEK1 inhibitor) or PAF-C16 (ERK1/2 activator). j Collagen I, Collagen III, and phospho-β-catenin expression in Adjacent, Control, and Niche fibroblasts treated with rhCTGF with or without PD98059 or PAF-C16. All in vitro experiments are performed in three independent replicates. Adjacent, adjacent myometrium tissue of niche; Control, well-healed cesarean scar tissue; Niche, niche tissue; HUF, human uterine fibroblasts; siNC, NC-siRNA; siLRP1, LRP1-siRNA. Source data are provided as a Source Data file. Created in BioRender. He, C. (2025) https://BioRender.com/krpej6u --.

Article Snippet: Following transfection with LRP1 siRNA, NC siRNA, lrp1 siRNA or nc siRNA for 48 hours, the cells were subsequently incubated with rhCTGF (HY- P70106 , MCE), PD98059 (HY-12028, MCE), or C16-PAF (HY-108635, MCE) for 48 hours.

Techniques: Derivative Assay, Control, Recombinant, Western Blot, Expressing, Transfection, In Vitro

Journal: Nature Communications

Article Title: Role of CTGF-LRP1 in impaired healing of cesarean section incisions

doi: 10.1038/s41467-026-69747-9

Figure Lengend Snippet: a Experimental timeline for the establishment and treatment of the rat uterine scar model. b Schematic illustration and gross anatomical image of the rat uterine scar model. c H&E staining of uterine horns on days 14 and 30 post-surgery across sham, PBS, and rhctgf-treated groups ( n = 8 uterine horns per group). Red arrows indicate scar repair sites. Scale bars = 500 μm. d Immunohistochemical staining for α-smooth muscle actin (α-SMA) at incision sites on days 14 and 30. Red arrows mark the repaired myometrial regions. Scale bars = 500 μm. The regenerating myometrium originated from residual tissue flanking the incision and appeared thin, irregular, and fragmented. e Masson’s trichrome staining assessing collagen deposition at incision sites on days 14 and 30. Scale bars = 200 μm. Quantitative analysis of collagen fiber area was performed. f , g Efficiency of lrp1 knockdown in rat uterine fibroblasts assessed at the mRNA level. RT-qPCR and Western blot analyses show Collagen I and III expression in fibroblasts transfected with nc-siRNA or lrp1-siRNA and treated with rhctgf. Data are shown as mean ± SEM. The p values were calculated using a two-tailed unpaired Student’s t -test. Source data are provided as a Source Data file. Created in BioRender. He, C. (2025) https://BioRender.com/krpej6u .

Article Snippet: Following transfection with LRP1 siRNA, NC siRNA, lrp1 siRNA or nc siRNA for 48 hours, the cells were subsequently incubated with rhCTGF (HY- P70106 , MCE), PD98059 (HY-12028, MCE), or C16-PAF (HY-108635, MCE) for 48 hours.

Techniques: Staining, Immunohistochemical staining, Knockdown, Quantitative RT-PCR, Western Blot, Expressing, Transfection, Two Tailed Test

Journal: Journal of Tissue Engineering

Article Title: Therapeutic potential and mechanisms of umbilical cord mesenchymal stem cells differentiating into tendon cells and promotion of rotator cuff tendon-bone healing

doi: 10.1177/20417314251315185

Figure Lengend Snippet: Analysis of the induction effect of BMP-12, GDF-7, and CTGF on the differentiation of UCMSCs: (a) morphological changes of UCMSCs after induction treatment, (b) mRNA expression of MKX, SCX, and TNC detected by qPCR, (c) bands and protein expression of MKX, SCX, and TNC detected by WB, and (d) COL-I and COL-III expression detected by immunofluorescence (400×). ** p < 0.01 and *** p < 0.001 compared with the control group.

Article Snippet: Rat umbilical cord mesenchymal stem cells (UCMSCs, CP-R302, Pricella, China), 293T cells (Pricella, China), DMEM medium (L110KJ, Yuanpei, China), Fetal bovine serum(AC03L055, life-ilab, China), GDF-6 (HY-P79333, MedChemexpress, USA), GDF-7 (92004ES10, Yisheng, China), CTGF (HY-P72154, MedChemexpress Biotechnology, USA), GoldenstarTM RT6 cDNA Synthesis Kit Ver.2 (TSK302M, Tsingke, China), qPCR Mix (SYBR Green I) kit and PCR primer (TSE002, Tsingke, China), RIPA lysis buffer (Beyotime, China), TNC (A1927, abclonal, China), MKX (ab236400, abcam, UK), SCX (ab58655, abcam, UK), GAPDH (A19056, abcam, UK), Hes1 (ab71559, abcam, UK), Hras (ab32417, abcam, UK), GAPDH Rabbit mAb (A19056, abclonal, China), Lentiviral Packaging Kit (41102ES10, Yisheng, China), Fetal bovine serum (26050070, Thermo, USA), trypsin (LP0042, Thermo, USA), PBS (10010001, Thermo, USA).

Techniques: Expressing, Immunofluorescence, Control